RNA processing requires the removal of introns from pre-mRNAs, a crucial step carried out by the spliceosome. Small nuclear RNAs (snRNAs) are essential components of the spliceosome and are encoded by large, multi-copy gene families. Variants of snRNAs generated by these gene families can influence alternative splicing and determine tissue-specific splicing programs. However, identifying and assessing their expression levels on a genome-wide scale has been challenging due to the high similarity of snRNA sequences. To overcome this challenge, we conducted a study using RAMPAGE (Rapid Amplification of cDNA Ends) data to comprehensively profile the nascent transcription of spliceosomal snRNAs. We identified 79 snRNA variants expressed in 115 human biosamples with unique promoter architecture, bidirectional transcription, and nucleosome positioning. Knockdown of the ATF/CREB family transcription factors would attenuate their transcription. Notably, nascent snRNA variant transcripts exhibited a distinct landscape compared to their mature transcripts. Our findings shed light on the transcriptional properties of snRNA variants and their potential role in regulating tissue-specific splicing.